Molecular players of iodine metabolism and mechanism by which T3 hormone controls cellular metabolism.
Candidate will join efforts of an international collaboration. The PhD candidate will make attempt to express several proteins and will after a while focus on a few most promising targets for structural biology approaches. The candidate will learn to use informatic tools and gain lab experience: parallel cloning and expression in heterologous systems (E. coli, insect, mammalian), protein purification, crystal structure determination, biochemical and biophysical analysis, and more.
Molekularni metabolizem joda in mehanizmov preko katerih T3 hormon vpliva na celični metabolizem.
Kandidat bo vključen v mednarodno sodelavo. Kandidat bo poskušal izraziti več proteinov in se v teku študija osredotočil na proteine, ki se bodo izkazali kot najobetavnejši za strukturno karkterizacijo. Pri svojem delu bo uporabljal informacijska orodja za pripravo nabora proteinov in analiziranje rezultatov ter izvajal paralelno kloniranje in izražanje proteinov v heterolognih ekspresijskih sistemih , njihovo čiščenje, kristalizacijo, dolčevanje strukture in njihovo biokemijsko analizo.
Candidates should contact Dusan Turk by e-mail. (Please, attach c.v. and publications list.)