Molecular players of iodine metabolism and mechanism by which T3 hormon controls cellular metabolism.
Candiate will join efforts of an international collaboration. The PhD candidate will make attempt to express several proteins and will after a while focus on a few most promissing tragets for structural biology approaches. The candidate will learn to use informatic tools and gain lab experience: parallel clonig and expression in heterologous systems (E. coli, insect, mammalian), protein purification, crystal structure determination, biochemical an dbiophysical analysis, and more.
Molekularni metabolizm joda in mehanizmov preko katerih T3 hormon vpliva na clelicni metabolizem.
Kandidat bo vključen v mednarodn osodelavo. Kandidat bo poskušala izraziti več proteinov proteinov in se v teku študija osredotočila na proteine, ki se bodo izkazali kot najobetavnejši za strukturno karkterizacijo. Pri svojem delu bo uporabljala informacijska orodja za pripravo nabora proteinov in analiziranje rezultatov ter izvajala paralelno kloniranje in izražanje proteinov v heterolognih ekspresijskih sistemih , njihovo čiščenje, kristalizacijo, dolčevanje strukture in njihovo biokemijsko analizo.
Candidates should contact Dusan Turk by e-mail. (Attach the cv and publications list.)